Situations may exist in which it may not be accurate to estimate a PPP recipient's payroll expenses based on the amount of PPP loan received. SES - Senior Executive Service Pay Calculatorįor Macvector Inc, the calculation used to estimate payroll costs is shown below:.FWS - Federal Wage System Pay Calculator.The program is accompanied by a slim, 32-page booklet to orient new users and by a larger user guide filled with helpful tips and detailed information on the various analyses. MacVector has a copy protection device however, one may purchase a site license, monitored by a KeyServer, for a specified number of users. Users without a CD-ROM drive may install the program over a network from another computer. Both programs install from a single CD-ROM in minutes, and take about 19 megabytes of hard-disk space. Thus, if the sequence of a 2-kilobase complementary DNA was obtained in six sequencing runs of overlapping 400-base pair segments, the results could be imported into AssemblyLIGN, which would find the overlaps and assemble them into a single sequence. This module is useful for assembling short sequences obtained from various regions of a larger sequence into a single contiguous stretch, or contig. The newest version, MacVector 6.5, has just been released, and like earlier versions, it comes with a separate contig assembly module called AssemblyLIGN. All this and more can be done with the help of MacVector. If further sequencing or cloning is to be carried out, it might be necessary to design primers for sequencing or PCR, or to choose probes for library screening. Comparison of the peptide sequence so obtained against databases of known proteins and searches for protein motifs could shed light on the function of the gene that was originally sequenced. If a nucleic acid sequence appears to encode a protein, it is of interest to translate the sequence and to analyze the properties of the putative translation product. The typical molecular biologist might want to enter sequence data obtained in the laboratory, then determine if it encoded any proteins or contained any special sequence motifs or restriction sites, construct maps that display any or all of the features of interest, and carry out sequence similarity searches against known sequences. MacVector is a "full-service" sequence analysis application that enables molecular biologists to analyze DNA and protein sequences on their Macintosh or PowerPC computers. The choices are varied, and the programs available range from those with a single function, such as the design of primers for polymerase chain reaction (PCR), to multipurpose products. Like the sequencing techniques themselves, the software available to carry out sequence analysis has become increasingly powerful and swift. Nowadays the main task is not sequence determination it is sequence analysis, with the help of desktop programs. The sequences that now fill databases have been determined not only by the research groups involved in the major genome sequencing projects, but also piecemeal by individual researchers investigating their genes of interest. Improvements in sequencing methods since that time have led to the rapid sequencing of many millions of base pairs from the genomes of a variety of organisms. MacVector 6.5 Oxford Molecular Group $2950 $395/$795 (upgrade) When nucleic acid sequencing was in its infancy, the mere determination of the sequence of a stretch of DNA was news.
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